Mouse Pancreatic Cancer Cells Ovalbumin-Luciferase Dual-Labeled

Ovalbumin (OVA) is a classic model antigen extensively used in immunology research. Known for its defined antigenic epitopes and ease of purification, it is frequently used to simulate pathogens or tumor antigens to study immune response mechanisms. In the biomedical field, the primary applications of OVA include:

1. Immunogenicity Studies: As a T-cell-dependent antigen, OVA can activate specific T-cell responses. OVA-stably transfected cells are easily recognized by the immune system as "non-self," mimicking the immune response under specific antigen stimulation. It is used to study T-cell activation, proliferation, and function, as well as the antigen-presenting mechanisms of dendritic cells (DCs).
2. Construction of Tumor Immune Models: Labeling tumor cells with OVA to mimic Tumor-Specific Antigens (TSA) turns the tumor cells into targets for the immune system, aiding in tumor therapy research. In tumor-specific immunity studies, OVA cells are often used to investigate T-cell activation, expansion, and cytotoxicity—especially CD8+ T-cell responses. This model helps researchers understand how the immune system monitors and attacks tumor cells and how to enhance anti-tumor effects through immunotherapy strategies.
3. Evaluation of Novel Immunotherapies: Using OVA-specific T cells (such as OT-I/OT-II TCR transgenic T cells) to attack OVA-expressing tumor cells allows for the study of immune cell recognition and killing mechanisms.
4. Drug Screening and Evaluation: Used for testing the in vivo and in vitro efficacy of immune checkpoint inhibitors (e.g., PD-1/PD-L1 antibodies) and CAR-T cell therapies, accelerating the development of new immunotherapies.

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Mouse Pancreatic Cancer Cells (OVA-LUC Dual-Labeled)

Basic Cell Information

Product Introduction

The Pan02 (also known as Panc 02) mouse pancreatic cancer cell line was originally induced by implanting 3-methylcholanthrene (3-MCA) into the pancreas of C57BL/6 mice. These cells exhibit epithelial-like morphology and adherent growth. Pan02 is currently one of the most commonly used syngeneic transplant models for Pancreatic Ductal Adenocarcinoma (PDAC). Known for its high invasiveness and resistance to chemotherapy, it is widely used in research concerning the pancreatic tumor microenvironment, immunotherapy, and anti-tumor drug screening.

OVA-stably transfected cells are easily recognized by the immune system as non-self, simulating immune responses under specific antigen stimulation. In tumor-specific immunity research, Pan02-OVA cells are particularly useful for studying the challenges of T-cell activation and infiltration in "Cold Tumors," specifically the killing response of CD8+ T cells (e.g., OT-I T cells).

The Pan02-luciferase (LUC) labeling allows for real-time tracking of tumor growth and drug distribution in orthotopic or metastatic pancreatic models via in vivo bioluminescence imaging. Combined with the immunogenicity of the OVA antigen, this Pan02-OVA-luciferase model enables the simultaneous assessment of drug efficacy and immune cell infiltration. This multi-modal labeling reduces experimental complexity and enhances data comprehensiveness and reliability.

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• Pan02: Accelerate your PDAC research with our highly invasive Pan02 cell line. View Pan02

• Pan02-OVA-Luciferase: Maximize your data with Pan02-OVA-Luciferase dual-labeled cells, allowing you to simultaneously track tumor burden and evaluate antigen-specific immunotherapy efficacy. View Pan02-OVA-Luc

• Pan02-Luciferase: Visualize tumor progression and metastasis in real-time with Pan02-Luciferase cells. View Pan02-Luc

Receiving Guide

Handling Cryovials upon Arrival:

Culture Methods

1. Medium:
   RPMI-1640+10% FBS+1%P/S (optional); add appropriate antibiotics to maintain selection pressure (follow product sheet).
2. Initial Handling procedure:
   
   1. Pipette 6-7 mL of complete medium into a 15 mL centrifuge tube.
   2. Gently agitate the vial in a 37°C water bath to thaw. Please keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1-2 minutes).
   3. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol or wiping with an alcohol cotton pellet. All of the operations from this point on should be carried out in the hood and under strict aseptic conditions.
   4. Transfer the vial contents to the step 1 centrifuge tube containing 6-7 mL complete culture medium. And spin at 1000 rpm for 3 mins at room temp to collect the cells.
   5. Resuspend cell pellet with 1ml recommended complete medium. And dispense into a T25 flask (or 6 cm culture dish) containing 4 mL of complete medium. (Note: It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH 7.0 to 7.6.)
   6. Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
3. Passaging (Subculturing):
   
   1. When the cells are 80%-90% confluent, it is ready to passage. Remove and discard culture medium.
   2. Rinse the cells 1-2 times with 1xPBS (2-3mL for T25 flask, increase or decrease the amount needed proportionally for culture vessels of other sizes) to remove residual medium and serum.
   3. Add 1 mL of Trypsin-EDTA solution to T25 flask (increase or decrease the amount needed proportionally for culture vessels of other sizes) allow trypsin completely cover the cells, then place the flask into the incubator and incubate for 1-2 mins (if cells are hard to detach, allow appropriate extension of incubation), and observe cells under an inverted microscope until cell layer is dispersed. (Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.)
   4. Add 2 times of trypsin vloume of complete growth medium to stop digestion, and aspirate cells by gently pipetting.
   5. Transfer the cell suspension with a 10 mL pipette into a 50 mL centrifuge tube, rinse the residual cells from the flask with PBS , then collect to the centrifuge tube.
   6. Centrifuge at 1000 rpm for 5 mins at room temp. Then remove and discard the supernatant and resuspend the cells with 2 mL of complete medium.
   7. Dispense the cells into a suitable culture vessel containing corresponding amount of complete medium.
   8. Incubate cultures at 37°C.
4. Cryopreservation:
   When the cell growth is good, cell cryopreservation can be performed.
   
   1. Collect cells and cell culture medium, transfer them into sterile centrifuge tubes, centrifuge at 1000 rpm for 5 mins, discard the supernatant, wash with PBS, discard all PBS, add 1mL of serum to resuspend cells, and perform cell counting.
   2. Add freezing media (50% RPMI-1640 + 40% FBS+10% DMSO or Serum-free freezing media) according to the number of cells, with a cell density of 1x10^6~1x10^7/mL, gently mix, and freeze 1mL of cell suspension in each cryopreservation tube.
   3. Put the cryopreservation tube into a -80 ℃ refrigerator and transfer it to liquid nitrogen for storage after 24 hours.

Precautions