VLU3B019

NCI-H460-Luc

This NCI-H460 luciferase stable cell line is made by lentivirus. Luciferase is stably expressed, and the luciferase activity is validated. Please feel free to contact us for more information.

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Key Facts

Product name
NCI-H460-Luc
Catalog No.
VLU3B019
Form
Ship in dry ice
Note
FOR RESEARCH USE ONLY
Product size: 1*10^6 cells/vial
Product price: Please inquire
Lead time: 2-3 weeks

This NCI-H460 luciferase stable cell line is made by lentivirus. Luciferase is stably expressed, and the luciferase activity is validated. Please feel free to contact us for more information.

Key facts
Product name
NCI-H460-Luc
Catalog No.
VLU3B019
Shipping and Storage
Ship in dry ice, store in liquid nitrogen vapor phase
Description
NCI-H460 Luciferase Cell Line engineered for stable, high-efficiency expression of the luciferase gene.This advanced tool delivers exceptionally high imaging quality and precise, quantifiable luminescence, ensuring your data is both robust and publication-ready.
Designed for versatility, the NCI-H460 Luciferase Cell Line seamlessly adapts to a broad spectrum of applications—from promoter activity assays and mammalian two-hybrid studies to sophisticated in vivo imaging.
NCI-H460 cell line was established from the pleural fluid of a patient with large cell lung cancer. NCI-H460 cells express p53 mRNA, which is easily detectable and at a level comparable to that of normal lung cells, indicating no overall structural abnormalities in the DNA of NCI-H460 cells. NCI-H460 cells are positive for keratin and vimentin fiber staining; negative for neurofilament triplet protein.
Properties
Host cell line
Human large cell lung cancer cell line (NCI-H460)
Alias
NCI.H460; H460; H-460; NCIH460; NCI-HUT-460; NCI-460
Species
Homo sapiens, human
Tissue origin
Lung
Target
Firefly luciferase
Application
Lumi test: Substrate D-Luciferin
Method
Lentivirus tranduction
Form
Poly-clonal
Quality control
Lumi-signal test
STR profiling
Cell culture
Biosafety level
1
Adherent/suspension
Adherent
Morphology
Epithelial-like
Culture medium
RPMI-1640+10%FBS+2ug/ml puromycin
Freezing medium
90% FBS+10% DMSO

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Datasheet

Safety datasheet

Protocol

For further support, please contact us.

*Publications utilizing this product should include the following acknowledgment: "The NCI-H460-Luc (Cat. No. VLU3B019) was obtained from Vitro Biotech, Inc."

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