VWT1B459

2V6.11

2V6.11 cell line is available in-stock. This cell line is mycoplasma, fungi, yeast, bacteria tested. Good viability guaranteed!

Product Images

Key Facts

Product name: 2V6.11
Catalog No.: VWT1B459
Form: Ship in dry ice
Note: FOR RESEARCH USE ONLY

Product size: 1*10^6 cells/vial

Product price: Please inquire

Lead time: 1-2 weeks

2V6.11 cell line is available in-stock. This cell line is mycoplasma, fungi, yeast, bacteria tested. Good viability guaranteed!

Key facts

Product name

2V6.11

Catalog No.

VWT1B459

Shipping and Storage

Ship in dry ice, store in liquid nitrogen vapor phase

Description

The 2V6.11 cell line was derived from the human embryonic kidney cell line 293 [HEK-293] in 2001. The 293 [HEK-293] cells were transfected with the plasmid pVgRXR carrying a Zeocin-resistant marker to obtain 293pVgRXR cells. Subsequently, they were transfected with the plasmid pEKORF6 containing the Ad5 E4ORF6 gene encoding E4 24K. pEKORF6 was derived from the plasmid pIndHydro containing the hygromycin resistance gene, and the vector contained SV40 viral sequences. The 2V6.11 cell line was initially isolated by cloning rings from a culture medium containing hygromycin to obtain monoclonal cells. The human adenovirus E4 34 KDa protein induced and expressed by 2V6.11 cells can be detected by Western blotting; 2V6.11 cells can be used as a tool for studying adenovirus E4 oncogenes.

Properties

Host cell line

Human Embryonic Kidney Cell Line

Alias

2V6.11

Species

Homo sapiens, human

Tissue origin

Kidney

Target

N/A

Application

Cell line research

Method

N/A

Form

Mammalian cell line

Quality control

STR Profiling

Mycoplasma test

Cell culture

Biosafety level

Adherent/suspension

Adherent

Morphology

Culture medium

MEM+10% FBS＋1% P/S

Freezing medium

55% complete culture medium+40%FBS+5%DMSO

Downloads

Datasheet

Safety datasheet

Protocol

For further support, please contact us.

*Publications utilizing this product should include the following acknowledgment: "The 2V6.11 (Cat. No. VWT1B459) was obtained from Vitro Biotech, Inc."