Hela

Hela cell line is the first non-aneuploid epithelial-like cell line derived from human tissue and maintained in continuous culture. Hela cell line was established by G. D. Gey et al. in 1951 from the cervical cancer of a 31-year-old black woman. Upon re-examination of the original tissue section samples, Jones et al. diagnosed it as adenocarcinoma. It is known that this cell line contains human papillomavirus type 18 sequences and requires operation in a Level 2 biosafety cabinet. Hela cells are positive for keratin and have low expression of p53, but express normal levels of Prb (retinoblastoma tumor suppressor).